Multiple forms of human intestinal alkaline phosphatase: chemical and enzymatic properties, and circulating clearances of the fast- and slow-moving enzymes
Identifieur interne : 003766 ( Main/Exploration ); précédent : 003765; suivant : 003767Multiple forms of human intestinal alkaline phosphatase: chemical and enzymatic properties, and circulating clearances of the fast- and slow-moving enzymes
Auteurs : Komoda Tsugikazu [Japon] ; Sakagishi Yoshikatsu [Japon] ; Sekine Takamitsu [Japon]Source :
- Clinica Chimica Acta [ 0009-8981 ] ; 1981.
English descriptors
- Teeft :
- Acta, Adult enzyme, Adult intestine, Affinity chromatography, Alkaline, Alkaline phosphatase, Alkaline phosphatase activity, Alkaline phosphatase fractions, Alkaline phosphatases, Amino, Amino acid, Amino acid analyzer, Amino acid composition, Amino acids, Anal biochem, Anodal migration, Apparent subunit weight, Arch biochem biophys, Biochem, Biochem biophys acta, Biochim, Biochim biophys acta, Biol chem, Biomedical press, Biophys, Blood group, Blood group persons, Blood group substance, Boehringer mannheim, Bovine serum albumin, Carbohydrate, Carbohydrate composition, Carbohydrate content, Cellogel membrane zymogram, Chloroquine diphosphate, Clearance, Concanavalin, Concanavalin affinity chromatography, Cysteic acid, Desialylated, Desialylated liver enzyme, Disc electrophoresis, Electrophoresis, Enzymatic properties, Enzyme, Enzyme activity, Enzyme assays, Enzyme peak, Enzyme preparation, Fetal, Fetal enzyme, Filtration, Flow rate, Glycoprotein, Group antibody, High concentrations, Human adult, Human liver, Human liver enzyme, Hydrophobic, Hydrophobic peptide, Insoluble papain, Intestinal, Intestinal enzyme, Intestinal enzymes, Intestine, Komoda, Latter enzyme, Linear gradient, Liver enzyme, Lymph fluid, Main fraction, Many respects, Methods enzymol, Miles laboratories, Molecular weight, Molecular weight estimation, Multiple forms, Native enzyme, Organ distribution, Original level, Other hand, Papain, Papain digestion, Partial purification, Peptide, Phenyl phosphate, Phosphatase, Phosphatase activity, Phosphatase isoenzymes, Polyacrylamide, Polyacrylamide disc, Preparative disc electrophoresis, Previous paper, Proc nat1 acad, Protein staining, Same buffer, Same procedures, Sephadex, Sephadex column, Sialic acid content, Sialylation, Sigma chemicals, Similar results, Slight modification, Small intestine, Sodium dodecyl sulfate, Specific activities, Specific activity, Substrate specificity, Subunit, Table viii, Triton, Untreated enzyme.
Abstract
Abstract: Two forms of alkaline phosphatase (orthophosphoric monoester phosphohydrolase (alkaline optimum, EC 3.1.3.1) have been purified from human small intestine by column chromatography on DEAE-cellulose and tyraminyl derivative affinity gel, and by preparative disc gel electrophoresis. Intestinal phosphatases were electrohoretically separated into two components, fast- and slow-moving enzymes, with apparent molecular weights of 140000 and 168000 and with subunit weights of 68000 and 80000, respectively.Analyses of carbohydrate and amino acid revealed marked differences in the two enzymes. Enzymatic properties and affinities for an anti-blood group antibody were also found to differ. Papain digestion released a hydrophobic small peptide from the slow-moving enzyme and its enzymatic properties resembled those of the fast-moving enzyme.Circulating clearance (T12) of the slow- and fast-moving enzymes from adult intestine was found to be 7.5 h and 1.3 h, respectively; that of fetal intestinal enzyme was 2.8 h. Sialidase, sialidase/β-galactosidase, or sialidase/β-galactosidase/ N-acetyl-β-glucosaminidase treatment of the fetal enzyme reduced the value to about 40 min. Further, digestion with α-fucosidase, α-mannosidase or both restored it to nearly the original level.Organ distribution of injected 125I-labelled enzymes indicates that the desialylated hepatic enzyme was selectively distributed in liver, while the degalactosylated intestinal enzyme was incorporated into liver, lymph fluid, and small intestine. These results suggest that the pathway of circulating clearance of alkaline phosphatase has several routes.
Url:
DOI: 10.1016/0009-8981(81)90037-1
Affiliations:
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enzyme</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Two forms of alkaline phosphatase (orthophosphoric monoester phosphohydrolase (alkaline optimum, EC 3.1.3.1) have been purified from human small intestine by column chromatography on DEAE-cellulose and tyraminyl derivative affinity gel, and by preparative disc gel electrophoresis. Intestinal phosphatases were electrohoretically separated into two components, fast- and slow-moving enzymes, with apparent molecular weights of 140000 and 168000 and with subunit weights of 68000 and 80000, respectively.Analyses of carbohydrate and amino acid revealed marked differences in the two enzymes. Enzymatic properties and affinities for an anti-blood group antibody were also found to differ. Papain digestion released a hydrophobic small peptide from the slow-moving enzyme and its enzymatic properties resembled those of the fast-moving enzyme.Circulating clearance (T12) of the slow- and fast-moving enzymes from adult intestine was found to be 7.5 h and 1.3 h, respectively; that of fetal intestinal enzyme was 2.8 h. Sialidase, sialidase/β-galactosidase, or sialidase/β-galactosidase/ N-acetyl-β-glucosaminidase treatment of the fetal enzyme reduced the value to about 40 min. Further, digestion with α-fucosidase, α-mannosidase or both restored it to nearly the original level.Organ distribution of injected 125I-labelled enzymes indicates that the desialylated hepatic enzyme was selectively distributed in liver, while the degalactosylated intestinal enzyme was incorporated into liver, lymph fluid, and small intestine. These results suggest that the pathway of circulating clearance of alkaline phosphatase has several routes.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Tsugikazu, Komoda" sort="Tsugikazu, Komoda" uniqKey="Tsugikazu K" first="Komoda" last="Tsugikazu">Komoda Tsugikazu</name></noRegion><name sortKey="Takamitsu, Sekine" sort="Takamitsu, Sekine" uniqKey="Takamitsu S" first="Sekine" last="Takamitsu">Sekine Takamitsu</name><name sortKey="Yoshikatsu, Sakagishi" sort="Yoshikatsu, Sakagishi" uniqKey="Yoshikatsu S" first="Sakagishi" last="Yoshikatsu">Sakagishi Yoshikatsu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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